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Briefly, 100 ng of human Poly-A+ RNA >200 nt and 2 ng of ERCC pool 14 RNA were used in a random hexamer (Invitrogen) and oligo-dT (Invitrogen) primed reverse transcription with Superscript III (Invitrogen) reaction carried out in the presence of actinomycin D (Invitrogen). This surely decreases range, but also reduces the Rx's sensitivity to unwanted interference. "Improving" the antennas on these designs can punish you with more glitches.
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